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rabbit anti tipe2 monoclonal antibody  (Boster Bio)


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    Boster Bio rabbit anti tipe2 monoclonal antibody
    Primer sequences used for using reverse transcription polymerase chain reaction to validate the microarray analysis.
    Rabbit Anti Tipe2 Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+tipe2+monoclonal+antibody/Anti-TNFAIP8L2+Antibody/pmc04501664-102-5-29
    Average 90 stars, based on 11 article reviews
    rabbit anti tipe2 monoclonal antibody - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats"

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2015.2241

    Primer sequences used for using reverse transcription polymerase chain reaction to validate the microarray analysis.
    Figure Legend Snippet: Primer sequences used for using reverse transcription polymerase chain reaction to validate the microarray analysis.

    Techniques Used: Reverse Transcription, Polymerase Chain Reaction, Microarray, Sequencing

    Effect of TIPE2 on the expression of Bcl-2, Fas and FasL in thymus T cells from septic mice. Groups of mice were induced by caecal ligation and puncture for 24 h. Representative western blot showing the levels of Bcl-2, Fas and FasL protein expression in thymus T cells in septic mice. Control group (non-septic group): (A) TIPE2-deficient mice; (B) wild-type mice; (C) TIPE2-deficient mice; (D) wild-type mice. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.
    Figure Legend Snippet: Effect of TIPE2 on the expression of Bcl-2, Fas and FasL in thymus T cells from septic mice. Groups of mice were induced by caecal ligation and puncture for 24 h. Representative western blot showing the levels of Bcl-2, Fas and FasL protein expression in thymus T cells in septic mice. Control group (non-septic group): (A) TIPE2-deficient mice; (B) wild-type mice; (C) TIPE2-deficient mice; (D) wild-type mice. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.

    Techniques Used: Expressing, Ligation, Western Blot, Control

    TIPE2 increases the thymus T-lymphocyte apoptosis rate in septic mice. Groups of mice were challenged by caecal ligation and puncture. 24 h later, the thymus T-lymphocyte apoptosis rate was measured using flow cytometry followed by quantitative evaluation. Values are expressed as the mean ± standard error of the mean of three experiments. * P<0.05 vs. wild-type mice. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2.
    Figure Legend Snippet: TIPE2 increases the thymus T-lymphocyte apoptosis rate in septic mice. Groups of mice were challenged by caecal ligation and puncture. 24 h later, the thymus T-lymphocyte apoptosis rate was measured using flow cytometry followed by quantitative evaluation. Values are expressed as the mean ± standard error of the mean of three experiments. * P<0.05 vs. wild-type mice. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2.

    Techniques Used: Ligation, Flow Cytometry

    Effect of Rhodiola rosea extract on the expression of TIPE2, Bcl-2, Fas and FasL mRNA in the thymus of septic mice. Groups of mice were induced by caecal ligation and puncture for 24 h. Representative gels of reverse transcription polymerase chain reaction products showing the levels of TIPE2, Bcl-2, Fas and FasL mRNA expression in rats. (A) Normal control group; (B) sham operation group; (C) control group; (D) treatment group. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.
    Figure Legend Snippet: Effect of Rhodiola rosea extract on the expression of TIPE2, Bcl-2, Fas and FasL mRNA in the thymus of septic mice. Groups of mice were induced by caecal ligation and puncture for 24 h. Representative gels of reverse transcription polymerase chain reaction products showing the levels of TIPE2, Bcl-2, Fas and FasL mRNA expression in rats. (A) Normal control group; (B) sham operation group; (C) control group; (D) treatment group. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.

    Techniques Used: Expressing, Ligation, Reverse Transcription, Polymerase Chain Reaction, Control

    Rhodiola rosea extract suppresses the expression of TIPE2, Bcl-2, Fas and FasL in thymus T cells from septic mice. Groups of mice were treated with Rhodiola rosea extract and 8 h later challenged by caecal ligation and puncture for 24 h. The expression of TIPE2, Bcl-2, Fas and FasL protein was assessed using western blot analysis and quantified by densitometric analysis. Values are expressed as the mean ± standard deviation of three experiments. * P<0.05, P<0.01 vs. sham-operated group and normal control group; # P<0.05 vs. control group. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.
    Figure Legend Snippet: Rhodiola rosea extract suppresses the expression of TIPE2, Bcl-2, Fas and FasL in thymus T cells from septic mice. Groups of mice were treated with Rhodiola rosea extract and 8 h later challenged by caecal ligation and puncture for 24 h. The expression of TIPE2, Bcl-2, Fas and FasL protein was assessed using western blot analysis and quantified by densitometric analysis. Values are expressed as the mean ± standard deviation of three experiments. * P<0.05, P<0.01 vs. sham-operated group and normal control group; # P<0.05 vs. control group. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.

    Techniques Used: Expressing, Ligation, Western Blot, Standard Deviation, Control

    Related Articles

    Reverse Transcription:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.. The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.. The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).

    Polymerase Chain Reaction:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.. The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.. The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).

    Microarray:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.. The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.. The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).

    Sequencing:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.. The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.. The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).

    Expressing:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.. The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.. The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).

    Ligation:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.. The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.. The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).

    Western Blot:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.. The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.. The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).

    Control:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.. The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.. The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).

    Flow Cytometry:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.. The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.. The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).

    Standard Deviation:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.. The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.. The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).The secondary antibodies used were horseradish peroxidase (HRP)-linked goat anti-rabbit immunoglobulin G (IgG) (1:4,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA) and sheep anti-mouse IgG-HRP (1:8,000 dilution; Amersham Pharmacia Biotech).



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    Image Search Results


    Primer sequences used for using reverse transcription polymerase chain reaction to validate the microarray analysis.

    Journal: International Journal of Molecular Medicine

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats

    doi: 10.3892/ijmm.2015.2241

    Figure Lengend Snippet: Primer sequences used for using reverse transcription polymerase chain reaction to validate the microarray analysis.

    Article Snippet: The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.

    Techniques: Reverse Transcription, Polymerase Chain Reaction, Microarray, Sequencing

    Effect of TIPE2 on the expression of Bcl-2, Fas and FasL in thymus T cells from septic mice. Groups of mice were induced by caecal ligation and puncture for 24 h. Representative western blot showing the levels of Bcl-2, Fas and FasL protein expression in thymus T cells in septic mice. Control group (non-septic group): (A) TIPE2-deficient mice; (B) wild-type mice; (C) TIPE2-deficient mice; (D) wild-type mice. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.

    Journal: International Journal of Molecular Medicine

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats

    doi: 10.3892/ijmm.2015.2241

    Figure Lengend Snippet: Effect of TIPE2 on the expression of Bcl-2, Fas and FasL in thymus T cells from septic mice. Groups of mice were induced by caecal ligation and puncture for 24 h. Representative western blot showing the levels of Bcl-2, Fas and FasL protein expression in thymus T cells in septic mice. Control group (non-septic group): (A) TIPE2-deficient mice; (B) wild-type mice; (C) TIPE2-deficient mice; (D) wild-type mice. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.

    Article Snippet: The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.

    Techniques: Expressing, Ligation, Western Blot, Control

    TIPE2 increases the thymus T-lymphocyte apoptosis rate in septic mice. Groups of mice were challenged by caecal ligation and puncture. 24 h later, the thymus T-lymphocyte apoptosis rate was measured using flow cytometry followed by quantitative evaluation. Values are expressed as the mean ± standard error of the mean of three experiments. * P<0.05 vs. wild-type mice. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2.

    Journal: International Journal of Molecular Medicine

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats

    doi: 10.3892/ijmm.2015.2241

    Figure Lengend Snippet: TIPE2 increases the thymus T-lymphocyte apoptosis rate in septic mice. Groups of mice were challenged by caecal ligation and puncture. 24 h later, the thymus T-lymphocyte apoptosis rate was measured using flow cytometry followed by quantitative evaluation. Values are expressed as the mean ± standard error of the mean of three experiments. * P<0.05 vs. wild-type mice. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2.

    Article Snippet: The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.

    Techniques: Ligation, Flow Cytometry

    Effect of Rhodiola rosea extract on the expression of TIPE2, Bcl-2, Fas and FasL mRNA in the thymus of septic mice. Groups of mice were induced by caecal ligation and puncture for 24 h. Representative gels of reverse transcription polymerase chain reaction products showing the levels of TIPE2, Bcl-2, Fas and FasL mRNA expression in rats. (A) Normal control group; (B) sham operation group; (C) control group; (D) treatment group. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.

    Journal: International Journal of Molecular Medicine

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats

    doi: 10.3892/ijmm.2015.2241

    Figure Lengend Snippet: Effect of Rhodiola rosea extract on the expression of TIPE2, Bcl-2, Fas and FasL mRNA in the thymus of septic mice. Groups of mice were induced by caecal ligation and puncture for 24 h. Representative gels of reverse transcription polymerase chain reaction products showing the levels of TIPE2, Bcl-2, Fas and FasL mRNA expression in rats. (A) Normal control group; (B) sham operation group; (C) control group; (D) treatment group. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.

    Article Snippet: The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.

    Techniques: Expressing, Ligation, Reverse Transcription, Polymerase Chain Reaction, Control

    Rhodiola rosea extract suppresses the expression of TIPE2, Bcl-2, Fas and FasL in thymus T cells from septic mice. Groups of mice were treated with Rhodiola rosea extract and 8 h later challenged by caecal ligation and puncture for 24 h. The expression of TIPE2, Bcl-2, Fas and FasL protein was assessed using western blot analysis and quantified by densitometric analysis. Values are expressed as the mean ± standard deviation of three experiments. * P<0.05, P<0.01 vs. sham-operated group and normal control group; # P<0.05 vs. control group. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.

    Journal: International Journal of Molecular Medicine

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats

    doi: 10.3892/ijmm.2015.2241

    Figure Lengend Snippet: Rhodiola rosea extract suppresses the expression of TIPE2, Bcl-2, Fas and FasL in thymus T cells from septic mice. Groups of mice were treated with Rhodiola rosea extract and 8 h later challenged by caecal ligation and puncture for 24 h. The expression of TIPE2, Bcl-2, Fas and FasL protein was assessed using western blot analysis and quantified by densitometric analysis. Values are expressed as the mean ± standard deviation of three experiments. * P<0.05, P<0.01 vs. sham-operated group and normal control group; # P<0.05 vs. control group. TIPE2, tumor necrosis factor-α-inducible protein 8-like 2; Bcl-2, B-cell lymphoma 2; FasL, Fas ligand.

    Article Snippet: The primary antibodies used included rabbit anti-TIPE2 monoclonal antibody (1:200), rabbit anti-Fas monoclonal antibody (1:200), rabbit anti-FasL monoclonal antibody (1:200), mouse anti-Bcl-2 monoclonal antibody (1:200) and were purchased from Wuhan Boster Biological Technology, Ltd.

    Techniques: Expressing, Ligation, Western Blot, Standard Deviation, Control